Time PCR Analysis of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

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New multiplex standard PCR and quadruplex real-time PCR assays for one-tube detection of Phyllosticta citricarpa, Elsinoë fawcettii, Elsinoë australis, and Pseudocercospora angolensis in Citrus: improvement and validation

Phyllosticta citricarpa, Elsinoë fawcettii, Elsinoë australis, and Pseudocercospora angolensis are main pathogens of citrus crops worldwide and might trigger non-characteristic signs which will result in confusion relating to the causative agent. These fungi are topic to worldwide phytosanitary laws, and testing on fruits or leaves requires correct and easy-to-use instruments.

New multiplex standard PCR and real-time PCR assays have been developed right here to realize extremely correct simultaneous detection of all 4 fungal pathogens in fruit tissues. We designed new oligonucleotide mixtures for P. citricarpa, E. fawcettii, and E. australis and mixed them with already accessible primers and hydrolysis probes for use in both PCR assay. The restrict of detection for multiplex standard PCR was as little as 100 pg μL-1 for P. citricarpa, E. fawcettii, and E. australis and 10 pg μL-1 of goal DNA per response tube for P. angolensis.

 The quadruplex real-time PCR assay efficiently yielded repeatable constructive outcomes with as little as 242, 243, 241, and 242 plasmidic copies of goal DNA of P. citricarpa, E. fawcettii, E. australis, and P. angolensis, respectively. Furthermore, evaluation of 60 naturally contaminated citrus samples yielded 100% concordant outcomes by each assays. Our validation experiment revealed that the multiplex real-time PCR assay confirmed excessive specificity besides a cross-reaction with P. paracitricarpa DNA.

Sensitivity, repeatability, reproducibility, and robustness have been verified, and the assay could possibly be used following totally different DNA extraction procedures, supporting health for routine evaluation. These new multiplex instruments needs to be of nice curiosity as cost-effective options for regulatory authorities and diagnostic laboratories, enabling testing for 4 necessary pathogens in single-tube reactions. KEY POINTS: • Improvement of recent standard PCR and qPCR assays for 4 citrus pathogens. • Very low limits of detection have been discovered for multiplex standard PCR. • qPCR had excessive specificity, sensitivity, repeatability, reproducibility, and robustness.

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Rat Salivary Gland, Parotid Total RNA*

RR-316 0.05mg
EUR 160

Rat Salivary gland, Parotid Total Protein

RT-316 1mg
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RF-316 10 slides
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RP-316 10 slides
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Rat WS Salivary Gland, Parotid Total RNA*

RR-316-WS 0.05mg
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Rat WS Salivary gland, Parotid Total Protein

RT-316-WS 1mg
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RF-316-WS 10 slides
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RP-316-WS 10 slides
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Mouse CD1 Salivary Gland, Parotid Total RNA

MR-316 0.05mg
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Mouse C57 Salivary Gland, Parotid Total RNA

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Mouse Balbc Salivary Gland, Parotid Total RNA

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Mouse CD1 Salivary gland, Parotid Total Protein

MT-316 1mg
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Mouse BLC Salivary gland, Parotid Total Protein

MT-316-BLC 1mg
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Mouse C57 Salivary gland, Parotid Total Protein

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MF-316-BLC 10 slides
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Mouse C57 Salivary gland, Parotid Frozen Sections

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Mouse CD1 Salivary gland, Parotid Paraffin Sections

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57142-2 BV SALIVARY PAROTID 25 EA*

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41241-2 RB SALIVARY PAROTID Y 25 EA*

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41342-2 RB SALIVARY PAROTID MATR 25 EA*

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Monkey Salivary Gland, cDNA, Cynomolgus

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RF-317 10 slides
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Salivary Gland Membrane Lysate

XBL-10846 0.1 mg
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Description: Human salivary gland tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human salivary gland tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated salivary gland tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated salivary gland tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

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Description: Purified native Mouse Salivary Gland virus protein

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Description: Human Gland Salivary Tissue Lysate

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UT-317 1mg
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UF-317 10 slides
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Monkey Salivary gland Total Protein, Cynomolgus

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Mouse CD1 Salivary gland, Mandibular Paraffin Sections

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MP-317-BLC 10 slides
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Mouse C57 Salivary gland, Mandibular Paraffin Sections

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Mouse CD1 Salivary gland, Sublingual Paraffin Sections

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Mouse BLC Salivary gland, Sublingual Paraffin Sections

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Mouse C57 Salivary gland, Sublingual Paraffin Sections

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SEM and qRT-PCR revealed quercetin inhibits morphogenesis of Aspergillus flavus conidia by way of modulating calcineurin-Crz1 signalling pathway

Aspergillus flavus: exploits numerous mechanisms to outlive throughout publicity to antifungal brokers together with morphogenesis. Germination of dormant conidia includes cascades of reactions built-in into the signalling pathway. This examine paperwork the impact of phytochemical-quercetin on A. flavus throughout germination of conidia utilizing scanning electron microscopy (SEM).

Vital inhibition of conidial swelling of A. flavus compared to management was noticed at Four and seven h Quantitative real-time PCR for genes from calcium signalling pathway and heat-shock proteins household confirmed up-regulation of warmth shock (Hsp70 and Hsp90) and calcium signalling pathway genes (calcium-transporting ATPase and calmodulin) in response to quercetin at preliminary Four h compared to management pattern whereas up-regulation of Hsp70, calcineurin and transcription issue Crz1, have been noticed in each the handled samples. Gene encoding for calcium-kinase, cAMP, Rho-gdp, Plc and Pkc confirmed a constitutively increased stage of expression in quercetin-treated pattern compared to management at each time factors. These information confirmed a transparent response from genes encoding calcineurin-Crz1 signalling pathways and should discover its software within the screening of antifungal brokers.

Abbreviations: Hsp: Hear shock protein; MIC: Minimal Inhibitory Focus; SEM: Scanning Electron Microscopy; qRT-PCR: Quantitative Actual-Time Polymerase Chain Response.

Quantitative Actual-Time PCR Analysis of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

MicroRNAs (miRNAs) are single stranded, non-coding RNA molecules that sometimes regulate gene expression on the post-transcriptional stage by binding to partially complementary goal websites within the 3′ untranslated area (UTR) of messenger RNA (mRNA), which reduces the mRNA’s translation and stability.

The miRNA expression profiles in numerous organs and tissues of mice have been investigated, however customary strategies for the purification and quantification of miRNA in mouse kidney haven’t been accessible. We now have established an efficient and dependable methodology for extracting and evaluating miRNA expression in mouse kidney with renal interstitial fibrosis by quantitative reverse-transcription polymerase chain response (qRT-PCR).

The protocol required 5 steps: (1) creation of sham and unilateral ureteral obstruction (UUO) mice; (2) extraction of kidney samples from the UUO mice; (3) extraction of whole RNA, which incorporates miRNA, from the kidney samples; (4) complementary DNA (cDNA) synthesis with reverse transcription from miRNA; and (5) qRT-PCR utilizing the cDNA. Utilizing this protocol, we efficiently confirmed that in comparison with the controls, the expression of miRNA-3070-3p was considerably elevated and people of miRNA-7218-5p and miRNA-7219-5p have been considerably decreased within the kidneys of a mouse mannequin of renal interstitial fibrosis. This protocol can be utilized to find out the miRNA expression within the kidneys of mice with UUO.

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Monkeypox Virus Real Time PCR Kit

ZD-0076-01 25 tests/kit Ask for price
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Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

PDPS-AR064 1 unit Ask for price
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VetAlert Johnes Real Time PCR Kit

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BioRT Real Time RT-PCR Kit(SYBR Green)

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EUR 491.4

Baculovirus Penaei Real Time PCR Detection kit

TRI-N12S1 16T
EUR 289.8

qTOWER3 auto Real Time PCR system

AJ844-00603-2 each
EUR 55045

Canine Distemper virus Real Time RT-PCR Detection kit

TRI-L10M1 32T
EUR 705.6

Canine Distemper virus Real Time RT-PCR Detection kit

TRI-L10S1 16T
EUR 378

HEV I, II, III, & IV Real Time RT-PCR Kit

GWB-LRB035 25 tests Ask for price

HEV I, II, III, & IV Real Time RT-PCR Kit

GWB-LRB036 25 tests Ask for price

HBV Quantitative & YMDD Mutation Real Time PCR Kit

GWB-LRB015 25 tests Ask for price

HBV Quantitative & YMDD Mutation Real Time PCR Kit

GWB-LRB016 25 tests Ask for price

HBV Quantitative & YMDD Mutation Real Time PCR Kit

GWB-LRB017 25 tests Ask for price

HBV Quantitative & YMDD Mutation Real Time PCR Kit

GWB-LRB018 25 tests Ask for price

High Pathogenicity PRRSV Real Time RT-PCR Detection kit

TRI-L31M1 48T
EUR 1008

High Pathogenicity PRRSV Real Time RT-PCR Detection kit

TRI-L31S1 24T
EUR 529.2

Mycoplasma haemofelis Real Time PCR Detection kit

TRI-L17M1 32T
EUR 453.6

Mycoplasma haemofelis Real Time PCR Detection kit

TRI-L17S1 16T
EUR 252

HBV Adefovir-resistant Mutants Real Time PCR Kit

GWB-LRB019 25 tests Ask for price

HBV Adefovir-resistant Mutants Real Time PCR Kit

GWB-LRB020 25 tests Ask for price

CPV and FPV Real Time PCR Detection kit

TRI-L18M1 32T
EUR 453.6

CPV and FPV Real Time PCR Detection kit

TRI-L18S1 16T
EUR 252

HBV Genetype B,C&D Real Time PCR Kit

GWB-LRB023 25 tests Ask for price

HBV Genetype B,C&D Real Time PCR Kit

GWB-LRB024 25 tests Ask for price

Bordetella bronchiseptica Real Time PCR Detection kit

TRI-L08M1 32T
EUR 453.6

Bordetella bronchiseptica Real Time PCR Detection kit

TRI-L08S1 16T
EUR 252

Transfusion-transmitted Virus (TTV) Real Time PCR Kit

GWB-LRB041 25 tests Ask for price

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