Technical Information
Application Notes
- Chiral Separations – Fast, Direct, Efficient
- This brief paper highlights four chiral separations to illustrate the speed, resolution and sensitivity of the new generation of analytical SFC instruments. The Aurora SFC Fusion A5 used in this paper extends the separation range of an Agilent HPLC making it an ideal instrument for chiral analysis. Additionally, we show how the use of a temperature controlled column compartment offers a wider range of operating conditions and can contribute to reduced detector noise.
- Easy Method Transfer: Reducing Analysis Time
- It is often desirable to transfer a method from a long column, packed with larger particles, to a shorter column with smaller particles, to reduce analysis time. This is feasible and easy using the SFC Fusion A5.
- Enantiomeric Excess Determination: Preliminary Study
- This study demonstrates that technical advances in the SFC Fusion A5 permit quantitation, even when the lesser component is less than .1% of the main component. The ICH guidelines require quantitation of any impurity representing >0.1% of the total area. In this study we are using SFC UV DAD to look for trace components representing <0.05% of the total area.
- Fast SFC Analysis: Effect of Particle Size and Column Length on Speed
- Conventionally, analytical SFC is performed on 5µm particles with column dimensions in the order of 4.6mm ID and 25cm in length. While these dimensions provide fast and efficient separations, higher throughput work is possible. This briefing paper explores what is possible with modern column packing's and dimensions.
- Improved Chiral Dynamic Range and Reproducibility
- Best sensitivity and widest dynamic range ever recorded in SFC. These results indicate SFC is suitable for use in all aspects of analysis including chiral, trace metabolites, impurities, QA/QC, API, GLP, GMP, etc.
- New Standards for Efficiency, Reproducibility, and Speed in SFC
- The Aurora SFC Fusion A5 module allows HPLC users to routinely achieve “HPLC” like performance for a far broader range of separations than before. “Liquid” CO2 is an ideal mobile phase for an extensive range of separations — giving predictable, reliable and extremely efficient separations — all with simple and familiar methods development techniques.
- SFC < 2 µm For High Throughput SFC Analysis
- Throughput can be significantly increased by reducing the delay time between the end of one run and the injection of the next sample. In this note we demonstrate 10 chromatograms with 5 baseline separated components each, in a total of 6 minutes for all 10 injections. Each (isocratic) chromatogram is 0.25 minutes long; the preparation for the next injection takes 0.35 minutes, for a total cycle time of just under 0.6 min.
- Temperature Effects on Selectivity in Analytical SFC
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Temperature is known to be an effective tool for changing selectivity in complex
mixtures using supercritical fluid chromatography (SFC). An achiral,
22–component mix representing wide diversity and known to be extremely difficult
to resolve, was studied between 20 and 70°C using a fixed, doubling gradient.
This work demonstrates the use of temperature to change selectivity. Each of
the 22 components in the mix is partially resolved using a single gradient and a
single pressure at a variety of temperatures. The work is then extended to
observe the effect of temperature on the trace contaminants in the mixture.
– View full text of study - Ultra Fast SFC: Quarter Minute Analysis
- Here, Zorbax RX-Sil (bare silica) 1.8µm particles in a 4.6x50mm column (Agilent Technologies) are used to demonstrate high speed, intermediate efficiency with symmetrical peaks, using a test mix of small drug-like molecules.